Flow Cytometry

Laser Scanning Cytometry (LSC) system, created and animated by Derek R. Vardon.

http://www.youtube.com/watch?v=8RzVvBOsM_w&hl=en

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Pump Tadpole "and" Frogometer "used to monitor pollution of waters with GM claw frog tadpoles. Courtesy: watchfrog every France.

http://www.youtube.com/watch?v=BPpUyYAINdI&hl=en

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The efficacy of study showed stemenhance ™ triple. Volunteers rested for one hour prior to the creation of baseline. After the first blood samples were volunteers, stemenhance ™ or a placebo. After blood samples were taken 30, 60 and 120 minutes after intake of consumption. The number of stem cells has been called the analysis of blood samples with a scientific method, Fluorescence-Activated Cell Sorting (FACS) measurements. The graph shows thePlacebo group in red, the group stemenhance ™ in blue. Consumption stemenhance ™ triggered a significant 25-30% strength by increasing the number of stem cells.

http://www.youtube.com/watch?v=aaurdxX5pZo&hl=en

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Kanecki Associates Inc. released the new FCS CYTORPRO Quick Facs Cytometry Software now shipping! ** FREE sample of good news for PC users !***** Cytometry Software FCS CYTOPRO QUICK FACS 11-process 24-48000 filesperhour FCS CYTOPRO QUICK FACS DOWNLOAD FREE TRIAL TODAY Cytometry FCS Software Cyotopro Quick Facs KANECKI ASSOCIATES, No tax INC. License fees for the cytometry software! Increasedquality and productivity. 10000000 file with events, canprocess24, 000 -42000Samples for hours and keep the quality up to 5orbetter Sigma. Compare that with your research technologistperformingonly 100 samples / analysis now. 1. Works with FCS 3.0 in all modes of data points, such as floating, integer * 4, and ASCII. 2. Work with BD and Beckman / Coulter flow cytometer and cell sorter. 3. Compatible with FCS 2.0 files and flow cytometers and sorting of cells. 4. FCS 3.0 can read files up to 10 events with 40 parameters. 5.Easy to use, it takes three steps. Load file beforeprocess set list specifyfile gate. That's it. N. menubar JUST EASYCLICKING! 6. Collaboration tools to help you cut and paste the results to the results. Directlyasmean analysis results imprinted on histogram 7.Statistical, mode, median, and the ability to achieve these logmodeor linear mode, depending on the detector used. 8. To make plain vanilla coding for easy update and maintenance, the user's largest and the quality of software. Record tract 9.Proven in FACS, fluorescent…

http://www.youtube.com/watch?v=ur28WiA-Kpo&hl=en

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Find in this movies the ins and outs of the Luminex business model.

http://www.youtube.com/watch?v=r8zjBDFn6ho&hl=en

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http://www.youtube.com/watch?v=nAfL4FXju1s&hl=en

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This tutorial demonstrates the easiest and quickest ways to import sample files from the cytometer into flowjo’s analysis program.

http://www.youtube.com/watch?v=TZ6a_1fFOZM&hl=en

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The gap between the early stage funding needs of a startup company and the expectations of a typical venture-capital firm can prevent many innovations from growing beyond a concept into a commercial commodity. Because venture capitalists and angel investors tend to support products and services in intermediate, less-risky stages of development, numerous government and academic institutions have created “pre-seed” or gap funds to accelerate the creation of new companies and sustain developing companies through the research phase so their owners can focus on preparing their businesses for later-stage equity investments.

Los Alamos National Security, the public-private partnership that runs Los Alamos National Laboratory, sponsors the Northern New Mexico Connect Venture Acceleration Fund to support businesses with lab affiliations. While such funding is rarely enough to carry a company all the way to profitability, it bestows credibility, public exposure and access to venture firms and allows an entrepreneur to reach critical commercial milestones that demonstrate to a potential backer how the company plans to deliver an attractive return on an investment.

Taking technology to the people

Administered through Los Alamos National Security’s Northern New Mexico Connect program, the Venture Acceleration Fund invests up to $100,000 in projects that find commercial uses for technological innovations and that create or develop regional businesses. The Northern New Mexico Connect program’s mission is connecting entrepreneurs with customized resources to accelerate growth of Northern New Mexico companies. Since the Venture Acceleration Fund began in 2006, the fund has awarded $600,000 to six northern New Mexico startups that use LANL-licensed technology, including biotech, plasma and computer-modeling applications.

One startup, Los Alamos-based Acoustic Cytometry Systems, is developing a commercially marketable version of a portable, hand-held acoustic-flow cytometer invented at LANL. The apparatus counts and measures cells using acoustic waves that concentrate the cells into a stream for laser analysis. Money from the Venture Acceleration Fund allowed the company to produce a prototype cytometer that is more sensitive, compact, and rugged – and less expensive to make – than a conventional cytometer, and these improvements make the instrument ideal for medical research in less developed parts of the world.

“The technical success of the project has enabled ACS to grow to eight employees in less than a year and to secure critical seed funding from private seed investors,” said John Elling, the company’s founder, president and CEO.

The other five startups supported by the fund are:

- The Company for Information Visualization and Analysis, created in Santa Fe in 2006 to develop a commercial use for EpiCast, a modeling and simulation system developed at LANL to help epidemiologists understand the spread of avian flu in human populations.

- Packet Analytics Corporation, a Santa Fe-based company that developed a computer-security software called the Network Forensic Search Engine based on technologies developed at LANL. The system centralizes security information in computer networks so that security managers can quickly investigate suspicious incidents.

- Santa Fe’s APJeT Inc., which is developing a machine that uses atmospheric-pressure plasma technology licensed by LANL to make textiles resistant to water, stains and other substances using ionized gas rather than toxic chemicals.

- Knowledge Reef Systems Inc. of Santa Fe, a LANL spinoff that is creating a new Internet-based social network through which people can share knowledge and information.

- CNT Technologies Inc., based in Los Alamos, which produces yarns using carbon nanotubes. The technique produces yarns stronger than other materials of equal weight at speeds hundreds of times faster than other techniques.

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As science becomes more and more advanced with fertility methods, gender selection is becoming more socially acceptable and more widely available. But what does this phrase really mean? One hundred years ago, it would’ve meant trying to conceive at a certain point during the month or day, but today it typically means something else entirely. Currently, selecting your baby’s gender is either performed through a clinic or on your own, trying all natural methods. Here’s a look at a few of the more popular methods couples use to determine the sex of their baby:

With Help From Clinics: There are a variety of methods that fertility clinics use to have you determine the sex of your child. Prices vary greatly depending on the service, but usually run in the thousands of dollars. IVF / PGD (described below) is typically $3,000 – $4,000 plus the cost of implanting the embryo, which is in the $10,000 range and up.

Sperm Sorting By Travel Speeds: This is among the oldest and least accurate of the techniques. The theory behind this is that X and Y sperm travel at different speeds. In this method, the couple’s sperm is placed in a solution. The male sperm are said to travel to the bottom first. So, sperm which seemingly match the couple’s preference is collected and implanted into the female.

Sperm Sorting By Laser / Flow Cytometry: This method sorts the sperm with a laser and then stains it with a dye which is supposed to indicate differences in DNA. This method is said to be around 90 percent accurate for a baby girl and about 75 percent accurate for a baby boy.

IVF Preimplantation Genetic Diagnosis (PGD): By far the most expensive, IVF is also the most accurate. Once the egg has been fertilized by sperm in the laboratory, doctors can distinguish male and female embryos by biopsy and replace only embryos of the desired gender into the womb. If this results “take” and result in the birth of a baby, this method can be nearly 100 percent accurate.

Natural, Inexpensive Methods At Home: Because of the high cost of clinic procedures with no real guarantee (except with PGD/ IVF), many couples opt for natural methods that combine diet / PH alterations, ovulation / conception timing and intercourse positioning that favor the conception of the desired gender. Depending on compliance, the quality of the program, and the ability of the couple to determine and alter the factors involved, in my experience, these natural methods can also work.

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